Email Address

info@canadianpoultryresearchnetwork.ca

Our Location

Across Canada

Follow Us :

CpG-ODN increases the antimicrobial immunity and cellular metabolism in broiler chickens

2021 CPRF
Synthetic oligodeoxynucleotides (ODN) containing unmethylated cytosine phosphodiester guanine (CpG) motifs are potent immune stimulants that promote antimicrobial mechanisms of immune cells. Activated immune cells increase energy demand of immune cells for cell proliferation, migration and increase cell signaling. The objective of this study was to investigate antimicrobial mechanisms and associated cellular metabolic pathways of chicken immune cells responsible for immunoprotection against infectious diseases. CpG-ODN (TCGTCGTTGTCGTTTTGTCGTT2007) was used in all the experiments at the dose of 50μg/bird and control group received saline by the intramuscular (IM) route in 2-3 week old broiler chickens. We have modified an assay developed for mammalian species and measured oxidative burst, phagocytosis and degranulation of chicken heterophils. Moreover, a novel assay using Agilent Seahorse XFp analyzer was optimized to quantify real-time measurement of glycolysis and mitochondrial respiration at cellular level in birds. Birds received CpG-ODN increased their oxidative burst activity up to 50-60% compared to the control group (30-35%). Phagocytosis of CpG-ODN treated birds (35-40%) was higher compared to the control birds (20-25%). An elevated degranulation was detected in birds received CpG-ODN (35-38%) compared to the control group (12-14%). Results of cellular metabolism indicated increased extracellular acidification rate (ECAR) 230-250 [(pmol/min)], compared to the control group [100-140 (pmol/min)]. Birds received CpG-ODN had increased mitochondrial respiration [oxygen consumption rate (OCR)] 70-80 (pmol/min) compared to the OCR in the control group 20-40 (pmol/min). Results of this study demonstrated that CpG-ODN mediated antimicrobial activity increased energy demand and metabolic activities of immune cells of broiler chickens. Novel assays that we developed will be very valuable in measuring metabolic activities of immune cells engaged with infectious disease.
Tags :
antimicrobial immunity,cellular metabolism
Share This :

Presenter

PhD Student

University of Saskatchewan

Follow on: