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Impact of barn bioaerosols on the health of poultry

2022 CPRF,Environment and Management
Poultry barn bioaerosols contain various gases, chemicals, dust particles and dead bacterial components in addition to live bacteria and other microbes. These particles such as bacterial components could induce inflammatory response in air ways. The environment also contains antibiotic resistant genes and some of them could be a great concern of public health. The current study is aimed at investigating the biotic (bacterial community) and abiotic factors (carbon dioxide (CO2), hydrogen sulphide (H2S), ammonium (NH4+), ammonia (NH3), NO2, nitrate (NO3-) and particle size) in layer chicken barns, its impact on the development of vaccine-mediated immune response and on the respiratory and overall health of the birds. We would aim to sample at least 10% of commercial layer operations (18/178). We will sample 3 free range organic (16%), 4 free runs (22%), 5 enriched (27%) and 6 conventional (35%) layer farms. We will measure the air quality near the bird level and our sampling will be done adjusting to bird’s head level using low-volume samplers. These samplers are capable of trapping biotic and abiotic constituents in the poultry barn environment at 5 particle size levels. We are particularly interested in bacteria present in the bioaerosols and their antibiotic sensitivity patterns and for which we will focus on characterizing 0.5-micron size filter trapped bacteria. Total microbial DNA will be extracted from the filter samples and used for 16S community analysis. Extracted DNA will be used for qPCR to determine the relative abundances and dynamics of select antibiotic resistance and mobile element target genes from samples. Based on our experiment, we will divide farms into three different air quality i.e excellent, medium, and poor. We will choose 3 pullet flocks from each category to evaluate the serological response IB, ILT and ND vaccines from each category of farms to establish correlation of vaccine effectiveness to barn air quality.
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University of Calgary

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