Email Address

info@canadianpoultryresearchnetwork.ca

Our Location

Across Canada

Follow Us :

Production and purification of recombinant antigens : challenges and solutions

2022 CPRF,Health & Immunity
Since the discovery of vaccination by Edward Jenner in 1976 and the development of the first small pox vaccine 2 years later, this immunological prevention strategy never stopped evolving. Nowadays with the advancement of technologies such as DNA sequencing and bioinformatics tools, a new era of vaccination, called reverse vaccinology, is available. This novel approach allows the identification of all surface antigens encoded by the genetic material of an microorganism. Because this approach is more accurate, faster and less expensive, it has quickly become a method used by many researchers. However, predicted antigens need to be assessed for their in vivo immunogenicity and as easy as this method seems, there are many challenges to overcome when it comes to protein production and purification. Different parameters such as host organisms, expression vectors, inclusion bodies or purification methods are critical to obtain an adequate recombinant protein yield. Once purified, different cleaning and cleaving steps need to be done prior to in vivo experiments to ensure that the observed immunogenicity can be associated to the recombinant protein. In this review, challenges and solutions regarding the production and the purification of recombinant antigens will be discussed.
Tags :
DNA sequencing,Health,Immunity,Recombinant Antigens
Share This :

Presenter

PhD Candidate

Université de Montréal

Follow on: